AsepticSure Research and Awards



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AsepticSure® Research and Awards



In-print December 2010

Peer reviewed by The American Journal of Infection Control

Effectiveness of a novel ozone-based system for the rapid high-level disinfection of health care spaces and surfaces

Methods: Ozone gas at 50-500 ppm was combined with 3% hydrogen peroxide vapor in a test chamber and upscaled in rooms measuring 82 m3 and 90 m3 in area. Test organisms included methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococcus, Escherichia coli, Pseudomonas aeruginosa, Clostridium difficile, and Bacillus subtilis spores dried onto steel discs or cotton gauze pads.


Results: The combination of 80-ppm ozone with 1% hydrogen peroxide vapor achieved a very high level of disinfection, with a 6 log10 reduction in the bacteria and spores tested on steel discs and MRSA tested on cotton gauze during a 30- to 90-minute exposure. The entire system was scalable such that it achieved the same high level of disinfection in both the 81-m3 and 90-m3 rooms in 60-90 minutes.


Dick Zoutman, MD, FRCPC, Michael Shannon, MD. MSc and Arkady Mandel, MD, PhD





July 7, 2011

AsepticSure™ Hospital Disinfection System Wins International Acclaim from World Health Organization

Medizone International’s AsepticSure™ hospital disinfection system receives innovation award at the World Health Organization First International Conference on Prevention and Infection Control (ICPIC)

– Medizone International, Inc. (OTCBB:MZEI) (OTCQB:MZEI)  proudly announces that its flagship disinfection technology, AsepticSure™, has won a prestigious international award for innovation. The award was received in a competition recently held at the World Health Organization’s first International Conference on Prevention and Infection Control (ICPIC) in Geneva, Switzerland.


Dick Zoutman, MD, FRCPC





June 12, 2012

Tuberculosis Breakthrough – New Technology To Greatly Reduce Deadly Disease Risk In Hospitals

Screenshot 2015-06-30 06.48.27AsepticSure, a Highly Promising New Disinfection Technology May Hold the Key to Reducing or Even Eliminating TB from Hospitals and Tuberculosis Laboratories, Worldwide.

San Francisco, California, Medizone International, Inc. (The Company) (MZEI:OB) (MZEI:QB) announced today that in achieving 100% kill rates with TB in three successive trials, it had achieved another important milestone in its understanding of the antimicrobial limits of its recently launched hospital disinfection technology, AsepticSure™.




Halifax May 2014

Canadian Infection Prevention And Control conference

 Screenshot 2015-06-29 15.08.06

Queen’s University Professor Emeritus Dick Zoutman, MD, FRCPC by Poster Presentation reported on a MRSA outbreak that began in May of 2013 on the Sills Ward of Bellville General Hospital in Bellville, Ontario, Canada

View The Presentation Poster from the Conference


Key Findings:                      

  • Historically the hospital reported the 20-bed ward averaged one to two new MRSA infections per month
  • Six patients had become infected and the ward was quarantined
  • Upon discharge the rooms and all equipment used by the infected patients such as wheel chairs and IV stands were disinfected, the outbreak was immediately quelled
  • In a follow up report it was confirmed that not only had the outbreak been fully contained, but the rooms then went MRSA free for 11 months

Financial Considerations: Using US CDC figures suggesting an average cost of $26,000 to treat every new HAI, this hospital would have retained an estimated $429,000 by the avoidance of 1.5 HAIs a month x 11 months while only cleaning 6 rooms with AsepticSure.





Posted on August 4, 2014

The National Research Council of Canada Viral Testing Laboratory Confirms

MERS Coronavirus Defeated by AsepticSure

Screenshot 2015-06-29 15.16.53

“Medizone has now completed multiple decontamination runs with well characterized samples of Adenovirus and MERS Coronavirus,” stated Dr Michael Shannon, Medizone’s President. “We are pleased to announce that each run has shown 100% kill in a full-scale room setting. Since all of the virology work has been carried out by an internationally respected team of virologists, we have complete confidence that these findings are correct. By destroying both of these viruses, one a non-enveloped virus and the second an enveloped Coronavirus, our understanding of the biological limits for AsepticSure® has been expanded dramatically and of course so has the range of medical applications.”





September 2014

AsepticSure Environmental Safety Report

A report produced by Walsh & Associates, a professional safety services company working in conjunction with the Analytical Services Unit of Queen’s University titled Report for Ozone Hydrogen Peroxide Industrial Hygiene Environmental Monitoring was commissioned.


The report, provided to the United States EPA, compared results to the following occupational exposure limits and environmental criteria: the Ontario Regulation respecting Control of Exposure to Biological or Chemical Agents (O. Reg. 833) TimeWeighted Average (TWA), ShortTerm Exposure Limit (STEL), and Excursion Limit; the U.S. Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit TWA (PELTWA); the American Conference of Governmental Industrial Hygienists (ACGIH®) Threshold Limit Value TWA (TLV®TWA) and Excursion Limits; the Ontario Ambient Air Quality Criteria (AAQC); Health Canada’s published Lowest Observed Adverse Effect Level (LOAEL); and the U.S. Environmental Protection Agency (EPA) National Ambient Air Quality Standards (NAAQS).

The Report confirmed that the moment the door to the AsepticSure disinfected room was opened following the disinfection process the room was safe and immediately available for occupancy.




May 2015

Hospital Rooms Cleaned to Autoclave Standards


The same FDA recommended biological indicators used to confirm medical instrument sterilization, may now be used to confirm absolute bactericidal kill throughout an entire hospital room following an AsepticSure disinfection.

Spore test strips have become the reference standard for routine quality assurance of hospital instrument sterilization and, according to the FDA, they should be utilized with each autoclave load. The same test strips are now proving a useful tool for confirming the same level of disinfection in an AsepticSure disinfected room (> 6-log) the same level of disinfection required for medical instrument sterilization.





Victoria June 2015

Canadian Infection Prevention and Control Conference


Queen’s University Professor Emeritus Dick Zoutman, MD, FRCPC by Poster Presentation reported on a C.difficile outbreak that began late November of 2014 on an acute care in-patient ward at Trenton Memorial Hospital, Ontario, Canada

Key Findings:

  • 4 infected patients on the 25-bed ward with one mortality
  • The outbreak was immediately contained following AsepticSure
  • The ward remained C.difficile free to the conference date
  • A time and cost comparison between conventional isolation room cleaning practices and AsepticSure found a time savings of 2 hours and 20 minutes over the four rooms in AsepticSure’s favor. Supply costs per room for AsepticSure were $3 per room, $7 for the manual method.



The Unique Superiority of AsepticSure® Disinfection

Facts and Conclusion

*          Using any other conventional double cleaning practices or disinfection technology it is simply not possible to achieve even a 3-log (99.9%) bactericidal and virucidal kill throughout the entire room. AsepticSure achieves > 6-log (> 99.9999%) kill of all pathogens, a thousand times more than 3-log.

*          Why is it necessary to achieve this absolute level of disinfection? Because in laboratory we have proven that following a 3-log, 99.9% kill, the remaining 0.1% of bacteria regenerates to full strength again in five days or less. This explains a major causative factor for the on-going HAI problem in health care facilities. Current practices simply do not eliminate all of the infective pathogens and they then regenerate, providing a reservoir capable of infecting future staff and patients.

*          It has now been demonstrated on numerous occasions in two hospitals during outbreaks of Pseudomonas aeruginosa, Methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile that following an AsepticSure disinfection the re-infection cycle has been broken. Rooms that previously had proven highly problematic, with higher incidence of patients acquiring infections while in hospital, became the most incident free rooms relative to HAI in the facility. In each case, one AsepticSure disinfection treatment was all that was required.

*          The cost savings to the medical system is unique. The United States CDC places the cost of treatment for each new HAI at $26,000. The annual cost of treating HAI in US hospitals has been estimated to be between $35.7B to $45.0B annually. Data gathered thus far on demonstrated HAI avoidance following the use of AsepticSure suggests the acquisition cost of the system, if only employed for isolation rooms following patient discharge, would be fully recaptured in as little as three months and a maximum of six months.

*          Recent time correlation studies demonstrated that isolation cleaning of 4 patient rooms with AsepticSure actually saved 2 hours and 20 minutes over conventional isolation room cleaning practices.


Save Lives

   Reduce Suffering

    Create a Safer Working Environment

       Greatly Reduced Costs Through Infection Avoidance